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* One more small cleanup, forgotten yesterday.Mathieu Arnold2021-04-071-1/+0
| | | | Reported by: lwhsu
* Remove # $FreeBSD$ from Makefiles.Mathieu Arnold2021-04-061-1/+0
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* biology/pooler: Upgrade to 1.76Jason W. Bacon2021-01-082-4/+4
| | | | | | | | | | Fixes race condition for make jobs in upstream Makefile PR: 252352 Submitted by: silas-freebsd@flatline.org.uk Notes: svn path=/head/; revision=560817
* biology/pooler: Optimise DNA sequencing primer-set combinationsJason W. Bacon2020-11-153-0/+51
Optimise combinations of primers and minimise the formation of dimers in multiplexed PCR. Primer Pooler can: * Check through each proposed pool for combinations that are likely to form dimers * Automatically move prospective amplicons between proposed pools to reduce dimer formation * Automatically search the genome sequence to find which amplicons overlap, and place their corresponding primers in separate pools * Optionally keep pool sizes within a specified range * Handle thousands of primers without being slow (useful for high-throughput sequencing applications) * Do all of the above with degenerate primers too. WWW: http://ssb22.user.srcf.net/pooler/ PR: ports/251065 Submitted by: Silas S. Brown <silas-freebsd@flatline.org.uk> Notes: svn path=/head/; revision=555418