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author | Jason W. Bacon <jwb@FreeBSD.org> | 2020-11-15 18:50:17 +0000 |
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committer | Jason W. Bacon <jwb@FreeBSD.org> | 2020-11-15 18:50:17 +0000 |
commit | 018d7f39c9b4d74b52faa6df26b805b5d61e7002 (patch) | |
tree | 7afffe915f08b699b48e8d7e9015706011602aa3 /dns/powerdns | |
parent | bf689b58be54f0891487378c04ee69b443c95ca1 (diff) | |
download | ports-018d7f39c9b4d74b52faa6df26b805b5d61e7002.tar.gz ports-018d7f39c9b4d74b52faa6df26b805b5d61e7002.zip |
biology/pooler: Optimise DNA sequencing primer-set combinations
Optimise combinations of primers and minimise the formation of dimers in
multiplexed PCR.
Primer Pooler can:
* Check through each proposed pool for combinations that are likely to form
dimers
* Automatically move prospective amplicons between proposed pools to reduce
dimer formation
* Automatically search the genome sequence to find which amplicons overlap, and
place their corresponding primers in separate pools
* Optionally keep pool sizes within a specified range
* Handle thousands of primers without being slow (useful for high-throughput
sequencing applications)
* Do all of the above with degenerate primers too.
WWW: http://ssb22.user.srcf.net/pooler/
PR: ports/251065
Submitted by: Silas S. Brown <silas-freebsd@flatline.org.uk>
Notes
Notes:
svn path=/head/; revision=555418
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